2011考研细胞生物学专业测试题附答案
一、填空题
1 . Cyclic AMP is produced by the enzyme________and degraded by the enzyme________
2 . NO是一种气体信号分子,在体内主要由精氨酸产生, 它的半衰期很短, 故只作用于邻近细胞, 它作用的靶酶是________
3. ________junctions enable cells to get a hold on the extracellular matrix by connecting their actin filaments to the matrix.
4 动物细胞质膜靠________建立膜两侧的质子动力势,而植物细胞则是靠________。
5. 蛋白质合成时多聚核糖体的形成对生命活动的意义在于:________。
二、判断题
1. Active transport is always driven by the hydrolysis of high-energy phosphate bonds.( )
2. Treatment of an animal cell with an inhibitor that is specific for the Na+/K+ pump is not likely to affect the uptake of glucose by sodium cotransport.( )
3. Tight junctions get their name from their property of holding cells togetherso tightly that they cannot be separated by mechanical forces.( )
4. Gap junctions connect the cytoskeletal elements of one cell to a neighboring cell or to the extracellular matrix.( )
5. NO acts only locally because it has a short half-life--about 5 to 10 seconds--in the extracellular space before it is converted to nitrates and nitrites by oxygen and water.( )
三、选择题
1. Which one of the following was used to determine the structure of the DNA molecule?( )
a. Transmission electron microscope b. Scanning electron microscope
c. Differential centrifugatioin d. X-ray crystallography
e. None of the above
2. You stain membrane proteins with a fluorescent-labeled antibody and notice the membrane is evenly stained. After a few hours, all of the fluorescence is at one end of a cell. You can conclude that( ).
a. Lipids flip-flop in the membrane. b. Proteins move laterally in the membrane.
c. The fluorescent dye was bleached. d. Proteins act as transporters.
e. All of the above
3. You have a preparation of ghosts and need to collect only the right-side out ghosts. You decide you can trap the right-side out ghosts in an affinity column packed with( )
a. SDS. b. A carbohydrate-binding molecule.
c. A protein-binding antibody. d. A serine-binding molecule.
e. None of the above
4. All of the following individuals contributed to cell theory except( )
a. Robert Hooke. b. Matthias Schleiden.
c. Theodor Schwann. d. Rudolf Virchow.
5. The addition of a peptide with an RGD sequence would probably ? the binding of cultured cells to a fibronectin coated dish.( )
a. enhance b. inhibit
c. have no effect on d. none of the above